研究業績

主な研究業績

Mori, T., Kumano, T., He, H., Watanabe, S., Senda, M., Moriya, T., S., Adachi, N., Hori, S., Terashita, Y., Kawasaki, M., Hashimoto, Y., Awakawa, T., Senda, T., Abe, I. & Kobayashi, M. “C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes”  Nature communications, in press (2021)

Kumano, T., Hori, S., Watanabe, S., Terashita, Y., Yu, H. Y., Hashimoto, Y., Senda, T., Senda, M. & Kobayashi, M. “FAD-dependent C-glycoside–metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis”  Proc. Natl. Acad. Sci. USA, 118, e2106580118 (2021)

Nagakubo, T., Kumano, T., Hashimoto, Y., & Kobayashi, M. “Copper amine oxidases catalyze the oxidative deamination and hydrolysis of cyclic imines”  Nature communications, 10, 413 (2019)

Nagakubo, T., Kumano, T., Hashimoto, Y., & Kobayashi, M. “Hemoglobin catalyzes CoA degradation and thiol addition to flavonoides”  Scientific Rep., 8, 1281 (2018)

Kumano, T., Fujiki, E., Hashimoto, Y. & Kobayashi, M., “Discovery of a sesamin-metabolizing microorganism and a new enzyme”  Proc. Natl. Acad. Sci. USA, 113, 9087-9092 (2016)

Abe, T., Hashimoto, Y., Zhuang, Y., Ge, Y., Kumano, T. & Kobayashi, M. “Peptide bond synthesis by a mechanism involving an enzymatic reaction and a subsequent chemical reaction” J. Biol. Chem., 291, 1735-1750 (2016)

Doi, S., Hashimoto, Y., Tomita, C., Kumano, T., & Kobayashi, M.,“Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde” Scientific Rep., 6, 38021 (2016)

Matsumoto, M., Hashimoto, Y., Saitoh, Y., Kumano, T. & Kobayashi, M. “Development of nitrilase promoter-derived inducible vectors for Streptomyces”  Biosci. Biotech. Biochem., 80, 1230-1237 (2016)

Nishiyama, T., Hashimoto, Y., Kusakabe, H., Kumano, T. & Kobayashi, M. “Natural low-molecular mass organic compounds with oxidase activity as organocatalysts”  Proc. Natl. Acad. Sci. USA, 111, 17152-17157 (2014)

Nomura, J., Hashimoto, H., Ohta, T., Hashimoto, Y., Wada, K., Naruta, Y., Oinuma, K. & Kobayashi, M. “Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis” Proc. Natl. Acad. Sci. USA, 110, 2810-2815 (2013)

Hassaninasab, A., Hashimoto, Y., Tomita-Yokotani, K. & Kobayashi, M. “Discovery of the curcumin metabolic pathway involving a unique enzyme in an intestinal microorganism”  Proc. Natl. Acad. Sci. USA, 108, 6615-6620, (2011)

Sato, H, Hashimoto, Y., Fukatsu, H. & Kobayashi, M. “Novel isonitrile hydratase involved in isonitrile metabolism”  J. Biol. Chem., 285, 34793-34802,(2010)

Zhou, Z, Hashimoto, Y., Cui, T., Washizawa, Y., Mino, H. & Kobayashi, M. “Unique biogenesis of high-molecular mass multimeric metalloenzyme nitrile hydratase: Intermediates, and a proposed mechanism for self-subunit swapping maturation” Biochemistry, 49, 9638-9648(2010)

Zhou, Z, Hashimoto, Y. & Kobayashi, M. “Self-subunit swapping chaperone needed for the maturation of multimeric metalloenzyme nitrile hydratase by a subunit exchange mechanism also carries out the oxidation of the metal ligand cysteine residues and insertion of cobalt”  J. Biol. Chem., 284, 14930-14938 (2009)

Zhou, Z, Hashimoto, Y., Shiraki, K. & Kobayashi, M. “Discovery of posttranslational maturation by self-subunit swapping”  Proc. Natl. Acad. Sci. USA, 105, 14849-14854 (2008)

Abe, T., Hashimoto, Y., Hosaka, H., Tomita-Yokotani, K. & Kobayashi, M. “Discovery of amide (peptide) bond synthetic activity in acyl-CoA synthetase”  J. Biol. Chem., 283, 11312-11321 (2008)

Sakashita, T., Hashimoto, Y., Oinuma, K-I. & Kobayashi, M. “Transcriptional regulation of the nitrile hydratase gene cluster in Pseudomonas chlororaphis B23”  J. Bacteriol., 190, 4210-4217 (2008)

小林達彦、他3名”新規物質TMR” 特許4912708号(特許成立)(2007)

Konishi, K., Ohta, T., Oinuma, K-I., Hashimoto, Y., Kitagawa, T. & Kobayashi, M. “Discovery of a reaction intermediate of aliphatic aldoxime dehydratase involving heme as an active center”  Proc. Natl. Acad. Sci. USA, 103, 564-568 (2006)

Hashimoto, Y., Hosaka, H., Oinuma, K-I., Goda, M., Higashibata, H. & Kobayashi, M. “Nitrile pathway involving acyl-CoA synthetase: Overall metabolic gene organization, and purification and characterization of the enzyme”      J. Biol. Chem., 280, 8660-8667 (2005)

Herai, S., Hashimoto, Y., Higashibata, H., Maseda, H., Ikeda, H., Omura, S. & Kobayashi, M. “Hyper-inducible expression system for streptomycetes”  Proc. Natl. Acad. Sci. USA, 101, 14031-14035 (2004)

Fukatsu, H., Hashimoto, Y., Goda, M., Higashibata, H. & Kobayashi, M. “Amine-synthesizing enzyme N-substituted formamide deformylase: Screening, purification, characterization and gene cloning”  Proc. Natl. Acad. Sci. USA, 101, 13726-13731 (2004)

Konishi, K., Ishida, K., Oinuma, K-I., Ohta,T., Hashimoto, Y., Higashibata, H., Kitagawa, T. & Kobayashi, M. “Identification of crucial histidines involved in carbon-nitrogen triple bond synthesis by aldoxime dehydratase”   J. Biol. Chem., 279, 47619-47625 (2004)

Oinuma, K-I., Hashimoto, Y., Konishi, K., Goda, M., Noguchi, T., Higashibata, H. & Kobayashi, M. “Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chlororaphis B23: Sequencing, gene expression, purification and characterization”  J. Biol. Chem., 278, 29600-29608 (2003)

Goda, M., Hashimoto, Y., Takase, M., Herai, S., Iwahara, Y., Higashibata, H. & Kobayashi, M. “Isonitrile hydratase from Pseudomonas putida N19-2: Cloning, sequencing, gene expression, and identification of its active amino acid residue”  J. Biol. Chem., 277, 45860-45865 (2002)

Goda, M., Hashimoto, Y., Shimizu, S. & Kobayashi, M. “Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage”  J. Biol. Chem., 276, 23480-23485 (2001)